Hotspot

rpl16

LSC · 67115-68301 · 1187 bp

Back to Scurrula

Pi 0.0656

MarkerSeek score 83.10

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1187
variable_sites: 198
pi: 0.0656
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	GGCATTGCGTCTTTGATCAC	ATCGAGTGGATCAGAGAGGG	50.0	55.0	58.1	58.0	1451-1790 bp	1.0	91.2
rpl16_p2	GGCATTGCGTCTTTGATCAC	GTCGGATCGACGGAAAAGAA	50.0	50.0	58.1	58.0	1480-1819 bp	1.0	91.1
rpl16_p3	GGCATTGCGTCTTTGATCAC	CTCCAGTGTCGACGGTATTG	50.0	55.0	58.1	58.1	1815-2154 bp	1.0	90.5
rpl16_p4	TTCTGGATCAACGACAACCG	ATCGAGTGGATCAGAGAGGG	50.0	55.0	58.2	58.0	1578-1887 bp	0.857143	84.6
rpl16_p5	TTCTGGATCAACGACAACCG	GTCGGATCGACGGAAAAGAA	50.0	50.0	58.2	58.0	1607-1916 bp	0.857143	84.5