Hotspot

trnN-GUU-trnR-ACG

IRa · 100723-101270 · 548 bp

Back to Scurrula

Pi 0.0387

MarkerSeek score 84.80

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 548
variable_sites: 62
pi: 0.0387
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	TGGTCGTAGGTTCGAATCCT	GGTACTCCCGGGAACAGATA	50.0	55.0	58.2	57.9	681-724 bp	1.0	89.2
trnN-GUU-trnR-ACG_p2	GCTGTTAACTGACTGGTCGT	GGTACTCCCGGGAACAGATA	50.0	55.0	57.8	57.9	694-737 bp	1.0	89.1
trnN-GUU-trnR-ACG_p3	CTGGTCGTAGGTTCGAATCC	GGTACTCCCGGGAACAGATA	55.0	55.0	57.8	57.9	682-725 bp	1.0	88.8
trnN-GUU-trnR-ACG_p4	GCTGTTAACTGACTGGTCGT	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	646-689 bp	1.0	88.5
trnN-GUU-trnR-ACG_p5	CTGTTCCTCAGTAGCTCAGC	GGTACTCCCGGGAACAGATA	55.0	55.0	57.7	57.9	727-770 bp	1.0	87.7