Hotspot

trnW-CCA-trnP-UGG

LSC · 53272-53385 · 114 bp

Back to Scurrula

Pi 0.0498

MarkerSeek score 82.60

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 114
variable_sites: 15
pi: 0.0498
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	TGCCGAACTGAACTAAGAGC	GGATGTAGCGCAGTTTGGTA	50.0	50.0	57.9	58.0	203-215 bp	1.0	87.3
trnW-CCA-trnP-UGG_p2	GTTTTGGAGACCCACGTTCT	GGATGTAGCGCAGTTTGGTA	50.0	50.0	58.0	58.0	222-234 bp	1.0	87.0
trnW-CCA-trnP-UGG_p3	TTTTGGAGACCCACGTTCTG	GGATGTAGCGCAGTTTGGTA	50.0	50.0	58.0	58.0	221-233 bp	1.0	87.0
trnW-CCA-trnP-UGG_p4	GTTTTGGAGACCCACGTTCT	GTTTGGTAGCGCGTTTGTTT	50.0	45.0	58.0	58.2	210-222 bp	1.0	86.2
trnW-CCA-trnP-UGG_p5	TGGAATCACGCTCTGTAGGA	GGATGTAGCGCAGTTTGGTA	50.0	50.0	58.2	58.0	261-273 bp	1.0	85.0