Hotspot

accD-psaI

LSC · 47678-48153 · 476 bp

Back to Scurrula

Pi 0.0774

MarkerSeek score 81.10

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 476
variable_sites: 101
pi: 0.0774
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GACAGGATTACCGGAAGCTG	GCCATTGCAAATGCTGGAAT	55.0	45.0	58.1	58.0	1092-1184 bp	1.0	91.3
accD-psaI_p2	ACCCGTTTGATCGAGTATGC	GCCATTGCAAATGCTGGAAT	50.0	45.0	58.1	58.0	968-1060 bp	1.0	91.2
accD-psaI_p3	CTGAGCTTGATGCAAATGGC	GCCATTGCAAATGCTGGAAT	50.0	45.0	58.1	58.0	881-973 bp	1.0	91.1
accD-psaI_p4	ATCGAGTATGCTACCCACCA	GCCATTGCAAATGCTGGAAT	50.0	45.0	57.9	58.0	959-1051 bp	1.0	91.1
accD-psaI_p5	GGAAGTCTGAGCTTGATGCA	GCCATTGCAAATGCTGGAAT	50.0	45.0	57.9	58.0	887-979 bp	1.0	90.9