Hotspot

atpB-rbcL

LSC · 43410-44139 · 730 bp

Back to Scurrula

Pi 0.0488

MarkerSeek score 84.30

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 730
variable_sites: 98
pi: 0.0488
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ATTCGTCCGAGAGTTGCTTC	GCTTTGAATCCAACACCTGC	50.0	50.0	58.0	57.9	1095-1126 bp	1.0	91.3
atpB-rbcL_p2	TACATCCAATACCGGACCGA	GTAAGTCCATCGGTCCACAC	50.0	55.0	57.9	58.0	1045-1073 bp	1.0	91.3
atpB-rbcL_p3	ATTCGTCCGAGAGTTGCTTC	GTAAGTCCATCGGTCCACAC	50.0	55.0	58.0	58.0	1275-1306 bp	1.0	90.8
atpB-rbcL_p4	ATTCGTCCGAGAGTTGCTTC	CGATCAAGGCTGGTAAGTCC	50.0	55.0	58.0	58.1	1287-1318 bp	1.0	90.3
atpB-rbcL_p5	ATTCGTCCGAGAGTTGCTTC	CTCTCCAGCGCATAAATGGT	50.0	50.0	58.0	58.0	1697-1728 bp	1.0	89.3