Hotspot

psaA-ycf3

LSC · 35217-35887 · 671 bp

Back to Scurrula

Pi 0.0451

MarkerSeek score 82.20

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 671
variable_sites: 79
pi: 0.0451
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCATCAGCATGTAGGTTCCA	TGGTTGAAGATTACGAGGCG	50.0	50.0	58.0	58.0	820-873 bp	0.857143	82.8
psaA-ycf3_p2	TAATGGAACCAACCCGCAAA	TGGTTGAAGATTACGAGGCG	45.0	50.0	58.0	58.0	1195-1248 bp	0.857143	81.8
psaA-ycf3_p3	TAATGGAACCAACCCGCAAA	GGTTGAAGATTACGAGGCGT	45.0	50.0	58.0	58.0	1194-1247 bp	0.857143	81.8
psaA-ycf3_p4	ATGTATTTGATGACCCGCCC	TGGTTGAAGATTACGAGGCG	50.0	50.0	58.0	58.0	1310-1363 bp	0.857143	81.5
psaA-ycf3_p5	ATGTATTTGATGACCCGCCC	GGTTGAAGATTACGAGGCGT	50.0	50.0	58.0	58.0	1309-1362 bp	0.857143	81.5