Hotspot

trnS-UGA-psbZ

LSC · 29123-29457 · 335 bp

Back to Scurrula

Pi 0.098

MarkerSeek score 78.30

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 335
variable_sites: 81
pi: 0.098
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAACCACTCAGCCATCTCTC	AAACGGCTAATTGGAAAGCA	55.0	40.0	58.0	56.2	366-441 bp	1.0	77.6
trnS-UGA-psbZ_p2	CAACCACTCAGCCATCTCTC	AACGGCTAATTGGAAAGCAA	55.0	40.0	58.0	56.2	365-440 bp	1.0	77.6
trnS-UGA-psbZ_p3	GAGCTATCAACCACTCAGCC	AAACGGCTAATTGGAAAGCA	55.0	40.0	58.1	56.2	373-448 bp	1.0	77.3
trnS-UGA-psbZ_p4	GAGCTATCAACCACTCAGCC	AACGGCTAATTGGAAAGCAA	55.0	40.0	58.1	56.2	372-447 bp	1.0	77.3
trnS-UGA-psbZ_p5	GGAGCTATCAACCACTCAGC	AACGGCTAATTGGAAAGCAA	55.0	40.0	58.1	56.2	373-448 bp	1.0	77.3