Hotspot

trnT-GGU-psbD

LSC · 25436-26375 · 940 bp

Back to Scurrula

Pi 0.0592
MarkerSeek score 83.60
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
940
variable_sites
156
pi
0.0592
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 CGGATTTGAACCAGCGTAGA AGCTTGGGTGGGGTTAAAAG 50.0 50.0 58.0 58.0 2227-2409 bp 1.0 91.9
trnT-GGU-psbD_p2 CGGATTTGAACCAGCGTAGA AGTTTCTTCAGCTTGGGTGG 50.0 50.0 58.0 58.0 2236-2418 bp 1.0 91.9
trnT-GGU-psbD_p3 CGGATTTGAACCAGCGTAGA GCAAGTTCGAATTGACGCAA 50.0 45.0 58.0 58.0 1905-2087 bp 1.0 91.7
trnT-GGU-psbD_p4 CGGCTCATTCTAAATGGGCT AGCTTGGGTGGGGTTAAAAG 50.0 50.0 58.0 58.0 1731-1916 bp 0.714286 75.4
trnT-GGU-psbD_p5 CGGCTCATTCTAAATGGGCT AGTTTCTTCAGCTTGGGTGG 50.0 50.0 58.0 58.0 1740-1925 bp 0.714286 75.4