Hotspot

petN-psbM

LSC · 23064-23815 · 752 bp

Back to Scurrula

Pi 0.0526

MarkerSeek score 85.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 752
variable_sites: 109
pi: 0.0526
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 85.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCAGCCCCAGTAACGTTCTA	AGTTCAACGGGTTAGAGCAC	50.0	50.0	58.1	57.8	1918-2174 bp	1.0	90.1
petN-psbM_p2	TCAGCCCCAGTAACGTTCTA	CAATCCCGACGAAGCCAATA	50.0	50.0	58.1	58.1	1860-2116 bp	0.714286	79.6
petN-psbM_p3	TTTCCCCAGTTCAAATCCGG	CAATCCCGACGAAGCCAATA	50.0	50.0	58.1	58.1	1540-1806 bp	0.714286	79.5
petN-psbM_p4	TTTCAGCCCCAGTAACGTTC	CAATCCCGACGAAGCCAATA	50.0	50.0	58.1	58.1	1862-2118 bp	0.714286	79.3
petN-psbM_p5	GTATCATTTTGGCGGCATGG	CAATCCCGACGAAGCCAATA	50.0	50.0	57.9	58.1	1592-1858 bp	0.714286	79.0