Hotspot

trnC-GCA-petN

LSC · 22697-22973 · 277 bp

Back to Scurrula

Pi 0.0887

MarkerSeek score 77.50

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 277
variable_sites: 60
pi: 0.0887
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TCAGCCCCAGTAACGTTCTA	GCCACTGTGAACTCTCGAAT	50.0	50.0	58.1	57.9	1100-1155 bp	1.0	90.7
trnC-GCA-petN_p2	TCAGCCCCAGTAACGTTCTA	GATTGGGCTGTTGACAAACG	50.0	50.0	58.1	57.9	1162-1208 bp	0.857143	85.3
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	GATTGGGCTGTTGACAAACG	50.0	50.0	58.1	57.9	842-894 bp	0.857143	85.2
trnC-GCA-petN_p4	TCAGCCCCAGTAACGTTCTA	AGGACTTGATTGGGCTGTTG	50.0	50.0	58.1	58.1	1169-1215 bp	0.714286	77.5
trnC-GCA-petN_p5	TCAGCCCCAGTAACGTTCTA	GGACTTGATTGGGCTGTTGA	50.0	50.0	58.1	58.1	1168-1214 bp	0.714286	77.4