Hotspot

rpoB-trnC-GCA

LSC · 21791-22625 · 835 bp

Back to Scurrula

Pi 0.0525

MarkerSeek score 88.30

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 835
variable_sites: 107
pi: 0.0525
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 88.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCTCATTTCCATCCCAGAGC	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.0	58.1	746-919 bp	1.0	91.2
rpoB-trnC-GCA_p2	CCTCATTTCCATCCCAGAGC	AACTGGGGAAAGGGGATTTG	55.0	50.0	58.0	57.7	737-910 bp	1.0	89.2
rpoB-trnC-GCA_p3	CCTCATTTCCATCCCAGAGC	ATTTGAACTGGGGAAAGGGG	55.0	50.0	58.0	57.7	742-915 bp	1.0	89.2
rpoB-trnC-GCA_p4	TCCCTCATTTCCATCCCAGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.7	58.1	748-921 bp	1.0	89.1
rpoB-trnC-GCA_p5	CCTCATTTCCATCCCAGAGC	TGAACTGGGGAAAGGGGATT	55.0	50.0	58.0	58.5	739-912 bp	1.0	87.6