Hotspot

trnS-GCU-trnR-UCU

LSC · 5049-5441 · 393 bp

Back to Scurrula

Pi 0.0915

MarkerSeek score 77.90

Primer available yes

Label trnS-GCU-trnR-UCU

Feature Metadata

label_name: trnS-GCU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 393
variable_sites: 86
pi: 0.0915
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	TGTTTGGCAAGCTGCTCTAA	GTTTCGAGAGTAGGATCCGC	45.0	55.0	58.0	57.9	1793-1919 bp	1.0	90.9
trnS-GCU-trnR-UCU_p2	TTGTTTGGCAAGCTGCTCTA	GTTTCGAGAGTAGGATCCGC	45.0	55.0	58.0	57.9	1794-1920 bp	1.0	90.9
trnS-GCU-trnR-UCU_p3	ATTAGCAATCCGCCGCTTTA	GTTTCGAGAGTAGGATCCGC	45.0	55.0	58.0	57.9	1129-1241 bp	1.0	90.7
trnS-GCU-trnR-UCU_p4	GCATCACTCGTACAACGGAT	GTTTCGAGAGTAGGATCCGC	50.0	55.0	58.1	57.9	1147-1259 bp	1.0	90.4
trnS-GCU-trnR-UCU_p5	TACGCATCACTCGTACAACG	GTTTCGAGAGTAGGATCCGC	50.0	55.0	58.1	57.9	1150-1262 bp	1.0	90.1