Hotspot

psbK-psbI

LSC · 4332-4759 · 428 bp

Back to Scurrula

Pi 0.099

MarkerSeek score 79.60

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 428
variable_sites: 110
pi: 0.099
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CCTTACCACTTGACTACGCC	TAAAGCGGCGGATTGCTAAT	55.0	45.0	58.0	58.0	1045-1211 bp	1.0	91.6
psbK-psbI_p2	CTTACCACTTGACTACGCCC	TAAAGCGGCGGATTGCTAAT	55.0	45.0	58.0	58.0	1044-1210 bp	1.0	91.6
psbK-psbI_p3	TGTTTGGCAAGCTGCTCTAA	TAAAGCGGCGGATTGCTAAT	45.0	45.0	58.0	58.0	602-728 bp	1.0	91.5
psbK-psbI_p4	TTGTTTGGCAAGCTGCTCTA	TAAAGCGGCGGATTGCTAAT	45.0	45.0	58.0	58.0	603-729 bp	1.0	91.5
psbK-psbI_p5	CTTACCACTTGACTACGCCC	CCATACCTATCCGGGCTGTA	55.0	55.0	58.0	58.1	1194-1361 bp	1.0	91.4