Hotspot

psbA-matK

LSC · 1247-1810 · 564 bp

Back to Scurrula

Pi 0.0475

MarkerSeek score 81.90

Primer available yes

Label psbA-matK

Feature Metadata

label_name: psbA-matK
feature_type: igs
region: LSC
length_bp: 564
variable_sites: 76
pi: 0.0475
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-matK_p1	TAAAGGCGGTTTTCAGTGCT	TGGCGCGAAGTCAAATGATA	45.0	45.0	58.0	58.0	1080-1177 bp	1.0	91.7
psbA-matK_p2	GTAAAGGCGGTTTTCAGTGC	TGGCGCGAAGTCAAATGATA	50.0	45.0	58.0	58.0	1081-1178 bp	1.0	91.5
psbA-matK_p3	CAAGAAAACAGCAGTCGCAG	TGGCGCGAAGTCAAATGATA	50.0	45.0	58.0	58.0	1471-1568 bp	1.0	89.7
psbA-matK_p4	TAAAGGCGGTTTTCAGTGCT	ATGGAGGAGGAGCAAGTTCT	45.0	50.0	58.0	58.0	718-812 bp	0.857143	85.5
psbA-matK_p5	CAAGAAAACAGCAGTCGCAG	ATGGAGGAGGAGCAAGTTCT	50.0	50.0	58.0	58.0	1109-1203 bp	0.857143	83.0