Hotspot

ycf1

SSC · 122079-126776 · 4698 bp

Back to Scrophularia

Pi 0.0109

MarkerSeek score 54.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 4698
variable_sites: 400
pi: 0.0109
unique_haplotype_count: 31
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGTGTTGTTCCAAATCGGTGA	TCGAATCTAAGTTGGAAGGGTC	42.9	45.5	58.1	57.5	208 bp	0.96875	58.4
ycf1_p2	TGAGTGTTGTTCCAAATCGGT	TCGAATCTAAGTTGGAAGGGTC	42.9	45.5	58.1	57.5	210 bp	0.96875	58.4
ycf1_p3	AGTGTTGTTCCAAATCGGTGA	CGAATCTAAGTTGGAAGGGTCT	42.9	45.5	58.1	57.5	207 bp	0.96875	58.4
ycf1_p4	TGAGTGTTGTTCCAAATCGGT	CGAATCTAAGTTGGAAGGGTCT	42.9	45.5	58.1	57.5	209 bp	0.96875	58.4
ycf1_p5	AGTGTTGTTCCAAATCGGTGA	TCGAATCTAAGTTGGAAGGGT	42.9	42.9	58.1	56.4	208 bp	0.96875	57.1