Hotspot

ndhA

SSC · 117989-120129 · 2141 bp

Back to Scrophularia

Pi 0.008

MarkerSeek score 56.50

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2141
variable_sites: 135
pi: 0.008
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGAGACAAGTTATGACGATGGA	TGGGAGAAGTTGATCGTTGA	40.9	45.0	56.9	56.5	2192-2221 bp	0.875	43.7
ndhA_p2	TGAGACAAGTTATGACGATGGA	TGGGAGAAGTTGATCGTTGAA	40.9	42.9	56.9	57.2	2192-2221 bp	0.875	41.7
ndhA_p3	TGAGACAAGTTATGACGATGGAA	TGGGAGAAGTTGATCGTTGA	39.1	45.0	57.6	56.5	2192-2221 bp	0.875	41.0
ndhA_p4	TTGAGACAAGTTATGACGATGGA	TGGGAGAAGTTGATCGTTGA	39.1	45.0	57.6	56.5	2193-2222 bp	0.875	41.0
ndhA_p5	TGAGACAAGTTATGACGATGGA	ATGGGAGAAGTTGATCGTTGA	40.9	42.9	56.9	57.0	2193-2222 bp	0.875	40.3