Hotspot

ndhD-psaC

SSC · 115407-115525 · 119 bp

Back to Scrophularia

Pi 0.0423

MarkerSeek score 67.70

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 119
variable_sites: 22
pi: 0.0423
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	GAACCCGCAAATATTGGCAA	ATGGTCTGAAACAACTCGCA	45.0	45.0	57.3	57.7	214-227 bp	0.96875	80.5
ndhD-psaC_p2	GAACCCGCAAATATTGGCAA	AAGAGATGTGAATCCGCCTG	45.0	50.0	57.3	57.7	267-280 bp	0.96875	79.7
ndhD-psaC_p3	GAACCCGCAAATATTGGCAA	GGTCTGAAACAACTCGCAGT	45.0	50.0	57.3	58.4	212-225 bp	0.96875	79.2
ndhD-psaC_p4	GAACCCGCAAATATTGGCAA	AAGAACCGAGGACTGTGTTG	45.0	50.0	57.3	57.8	292-305 bp	0.9375	79.1
ndhD-psaC_p5	GAACCCGCAAATATTGGCAA	CAAGAACCGAGGACTGTGTT	45.0	50.0	57.3	57.8	293-306 bp	0.9375	79.1