Hotspot

rpl32-trnL-UAG

SSC · 111635-112500 · 866 bp

Back to Scrophularia

Pi 0.0223

MarkerSeek score 70.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 866
variable_sites: 133
pi: 0.0223
unique_haplotype_count: 31
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TGGAAGTGGAAGGAAGGGTA	TGATCCACATAAAAGGGCCG	50.0	50.0	57.6	57.9	1838-2279 bp	1.0	88.2
rpl32-trnL-UAG_p2	TGGAAGTGGAAGGAAGGGTA	GATAGATATGCCGCCACTCG	50.0	55.0	57.6	58.1	1275-1716 bp	1.0	87.9
rpl32-trnL-UAG_p3	TGGAAGTGGAAGGAAGGGTA	AGCAAAGCCCTGGGTAAAAA	50.0	45.0	57.6	57.9	1715-2156 bp	1.0	87.8
rpl32-trnL-UAG_p4	TGGAAGTGGAAGGAAGGGTA	GTCCCGAATAAACCCAACGA	50.0	50.0	57.6	57.9	1569-2010 bp	1.0	87.8
rpl32-trnL-UAG_p5	TGGAAGTGGAAGGAAGGGTA	CCCGAATAAACCCAACGAGT	50.0	50.0	57.6	57.9	1567-2008 bp	1.0	87.8