Hotspot

ndhC-trnV-UAC

LSC · 50106-51203 · 1098 bp

Back to Scrophularia

Pi 0.0161

MarkerSeek score 68.30

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1098
variable_sites: 127
pi: 0.0161
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CTAGAAAGTTTCTCCGGCCC	TGAATCAGAGCACATGGAGC	55.0	50.0	58.0	58.0	1373-1447 bp	1.0	91.3
ndhC-trnV-UAC_p2	CTAGAAAGTTTCTCCGGCCC	TTGGTAGTGCTCCTGGATCA	55.0	50.0	58.0	58.0	1406-1480 bp	1.0	91.1
ndhC-trnV-UAC_p3	CTAGAAAGTTTCTCCGGCCC	CAAACAGACCCCACCATTGA	55.0	50.0	58.0	58.0	1638-1717 bp	1.0	90.7
ndhC-trnV-UAC_p4	GTCGCCCATCGGTTCTATAC	CAAACAGACCCCACCATTGA	55.0	50.0	57.9	58.0	1669-1748 bp	1.0	90.3
ndhC-trnV-UAC_p5	GGGGCTAAAATCCCGGAAAT	CAAACAGACCCCACCATTGA	50.0	50.0	57.9	58.0	1608-1687 bp	1.0	90.2