Hotspot

rps4-trnT-UGU

LSC · 45619-46004 · 386 bp

Back to Scrophularia

Pi 0.0198

MarkerSeek score 69.60

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 386
variable_sites: 45
pi: 0.0198
unique_haplotype_count: 31
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TAGTCCTGGTAAAACCCCCA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.6	57.9	499-542 bp	0.96875	85.3
rps4-trnT-UGU_p2	TAGTCCTGGTAAAACCCCCA	ATGGTCATCGGTTCGATTCC	50.0	50.0	57.6	57.8	467-510 bp	0.96875	84.9
rps4-trnT-UGU_p3	TAGTCCTGGTAAAACCCCCA	CTATTATGTGCGCCCGCTTA	50.0	50.0	57.6	58.2	520-563 bp	0.9375	83.3
rps4-trnT-UGU_p4	TAGTCCTGGTAAAACCCCCA	TATTATGTGCGCCCGCTTAG	50.0	50.0	57.6	58.2	519-562 bp	0.9375	83.3
rps4-trnT-UGU_p5	TAGTCCTGGTAAAACCCCCA	CCGCTTAGCTCAGAGGTTAG	50.0	55.0	57.6	57.5	507-550 bp	0.96875	82.4