Hotspot

psaA-ycf3

LSC · 41302-42039 · 738 bp

Back to Scrophularia

Pi 0.0147

MarkerSeek score 65.60

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 738
variable_sites: 74
pi: 0.0147
unique_haplotype_count: 29
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGTGAGTTGGATCGCTTAGC	TGGTAGAGGAAGCACCGTAA	50.0	50.0	58.0	58.1	1757-1884 bp	1.0	87.4
psaA-ycf3_p2	GCATCAGCATGTAGGTTCCA	TGGTAACGGGACACCAAAAG	50.0	50.0	58.0	58.0	1142-1269 bp	0.90625	84.7
psaA-ycf3_p3	TGTGAGTTGGATCGCTTAGC	TGGTAACGGGACACCAAAAG	50.0	50.0	58.0	58.0	1306-1433 bp	0.90625	84.0
psaA-ycf3_p4	GTTATTCCAGATGCTCGCCA	TGGTAACGGGACACCAAAAG	50.0	50.0	58.0	58.0	1436-1563 bp	0.90625	83.2
psaA-ycf3_p5	CCCCGCTAAATGGTGATTCA	TGGTAACGGGACACCAAAAG	50.0	50.0	57.9	58.0	1587-1714 bp	0.875	80.9