Hotspot

atpH-atpI

LSC · 12915-13938 · 1024 bp

Back to Scrophularia

Pi 0.0101

MarkerSeek score 67.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1024
variable_sites: 76
pi: 0.0101
unique_haplotype_count: 29
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GCCAATCCAGCAGCAATAAC	TCCCTGTCATGTTCCTTGGA	50.0	50.0	57.8	58.3	1128-1188 bp	0.96875	84.5
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TCCCTGTCATGTTCCTTGGA	45.0	50.0	58.0	58.3	1113-1164 bp	0.9375	84.4
atpH-atpI_p3	AATAACGGAAGCGGCAGAAA	TCCCTGTCATGTTCCTTGGA	45.0	50.0	58.2	58.3	1114-1165 bp	0.9375	83.2
atpH-atpI_p4	TCCAGCAGCAATAACGGAAG	TCCCTGTCATGTTCCTTGGA	50.0	50.0	58.3	58.3	1123-1183 bp	0.9375	82.7
atpH-atpI_p5	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.0	58.5	1065-1116 bp	0.875	79.7