Hotspot

trnK-UUU-rps16

LSC · 4252-4736 · 485 bp

Back to Scrophularia

Pi 0.0157

MarkerSeek score 80.30

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 485
variable_sites: 50
pi: 0.0157
unique_haplotype_count: 31
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	GTGCTCAACCCACAGGAAC	50.0	57.9	58.2	58.7	459-614 bp	1.0	76.5
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	GTGCTCAACCCACAGGAAC	50.0	57.9	58.2	58.7	458-613 bp	1.0	76.5
trnK-UUU-rps16_p3	TCTAGCCGCACTTAAAAGCC	GTGCTCAACCCACAGGAAC	50.0	57.9	58.3	58.7	473-628 bp	1.0	75.6
trnK-UUU-rps16_p4	TCAGTCGTGGTCTTCCAAAC	GTGCTCAACCCACAGGAAC	50.0	57.9	57.8	58.7	544-699 bp	1.0	75.5
trnK-UUU-rps16_p5	CAGTCGTGGTCTTCCAAACT	GTGCTCAACCCACAGGAAC	50.0	57.9	57.8	58.7	543-698 bp	1.0	75.5