Hotspot

trnH-GUG-psbA

LSC · 77-382 · 306 bp

Back to Scrophularia

Pi 0.0693

MarkerSeek score 75.40

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 306
variable_sites: 93
pi: 0.0693
unique_haplotype_count: 31
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GAGTGGGAGCTTAGTTTCCG	55.0	55.0	58.1	58.0	999-1196 bp	1.0	90.3
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	GAGCTTAGTTTCCGTCTGGG	55.0	55.0	58.1	58.0	993-1190 bp	0.96875	89.0
trnH-GUG-psbA_p3	GATCCACTTGGCTACATCCG	GGAGCTTAGTTTCCGTCTGG	55.0	55.0	58.1	58.0	994-1191 bp	0.96875	89.0
trnH-GUG-psbA_p4	GATCCACTTGGCTACATCCG	GGGAGCTTAGTTTCCGTCTG	55.0	55.0	58.1	58.0	995-1192 bp	0.96875	89.0
trnH-GUG-psbA_p5	CAATCCACTGCCTTGATCCA	GAGCTTAGTTTCCGTCTGGG	50.0	55.0	57.9	58.0	1007-1204 bp	0.96875	88.9