Hotspot

ndhK

LSC · 49051-49728 · 678 bp

Back to Scorzonera

Pi 0.0103

MarkerSeek score 50.70

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: LSC
length_bp: 678
variable_sites: 7
pi: 0.0103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	CCTCTTCTGGTTGATCCACG	ATAGAACCAATAGGCGACGC	55.0	50.0	58.0	57.8	1303-1304 bp	1.0	79.4
ndhK_p2	CCTCTTCTGGTTGATCCACG	TGGGCAATGAGTTTCGATGT	55.0	45.0	58.0	57.8	1201-1202 bp	1.0	79.1
ndhK_p3	CCTCTTCTGGTTGATCCACG	GTTTATGCATGGCGAAAGGG	55.0	50.0	58.0	57.8	1120-1121 bp	1.0	79.1
ndhK_p4	TAATAGTCCGCCTGGTGCTA	ATAGAACCAATAGGCGACGC	50.0	50.0	57.9	57.8	1248-1249 bp	1.0	79.0
ndhK_p5	CCTCTTCTGGTTGATCCACG	GAAACACGCCCGAAACTAGA	55.0	50.0	58.0	58.2	1575-1576 bp	1.0	78.8