Hotspot

rps12-rps7

IRa · 138878-138930 · 53 bp

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Pi 0.0189

MarkerSeek score 63.30

Primer available yes

Label rps12-rps7

Feature Metadata

label_name: rps12-rps7
feature_type: igs
region: IRa
length_bp: 53
variable_sites: 1
pi: 0.0189
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-rps7_p1	GGCTTGGAGGGAGATCTTTC	TTCTTCTGCAGTACCTCGAC	55.0	50.0	57.7	57.0	149 bp	1.0	69.7
rps12-rps7_p2	GGCTTGGAGGGAGATCTTTC	TCTTCTGCAGTACCTCGACG	55.0	55.0	57.7	59.2	148 bp	1.0	68.2
rps12-rps7_p3	TATGTACGGCTTGGAGGGAG	TTCTTCTGCAGTACCTCGAC	55.0	50.0	58.6	57.0	156 bp	1.0	67.7
rps12-rps7_p4	GGCTTGGAGGGAGATCTTTC	TTTCTTCTGCAGTACCTCGAC	55.0	47.6	57.7	57.7	150 bp	1.0	67.4
rps12-rps7_p5	GGCTTGGAGGGAGATCTTTC	CTTCTGCAGTACCTCGACG	55.0	57.9	57.7	57.7	147 bp	1.0	67.3