Hotspot

petD-rpoA

LSC · 76951-77162 · 212 bp

Back to Scorzonera

Pi 0.0283

MarkerSeek score 64.10

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 212
variable_sites: 6
pi: 0.0283
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	TTGGTTGGGTATTGGTGCAA	TGGAGCATTTTCGCATAGAAGA	45.0	40.9	57.9	58.1	362-376 bp	1.0	63.6
petD-rpoA_p2	TTTGGTTGGGTATTGGTGCA	TGGAGCATTTTCGCATAGAAGA	45.0	40.9	57.9	58.1	363-377 bp	1.0	63.6
petD-rpoA_p3	TTGGTTGGGTATTGGTGCAA	TGGAGCATTTTCGCATAGAAG	45.0	42.9	57.9	56.7	362-376 bp	1.0	62.1
petD-rpoA_p4	TTTGGTTGGGTATTGGTGCA	TGGAGCATTTTCGCATAGAAG	45.0	42.9	57.9	56.7	363-377 bp	1.0	62.1
petD-rpoA_p5	CCAGTAGCGACAACCGTATT	TGGAGCATTTTCGCATAGAAGA	50.0	40.9	57.7	58.1	406-420 bp	1.0	61.8