Hotspot

trnW-trnP

LSC · 65813-65976 · 164 bp

Back to Scorzonera

Pi 0.0488

MarkerSeek score 75.90

Primer available yes

Label trnW-trnP

Feature Metadata

label_name: trnW-trnP
feature_type: igs
region: LSC
length_bp: 164
variable_sites: 8
pi: 0.0488
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-trnP_p1	GTTTTGGAGACCCACGTTCT	GTCACGGGTTCAAATCCTGT	50.0	50.0	58.0	58.1	233 bp	1.0	81.6
trnW-trnP_p2	GTTTTGGAGACCCACGTTCT	CACGGGTTCAAATCCTGTCA	50.0	50.0	58.0	58.1	231 bp	1.0	81.6
trnW-trnP_p3	GTTTTGGAGACCCACGTTCT	TGTCACGGGTTCAAATCCTG	50.0	50.0	58.0	58.1	234 bp	1.0	81.6
trnW-trnP_p4	GTTTTGGAGACCCACGTTCT	GGGTACAAAATGTCACGGGT	50.0	50.0	58.0	58.1	244 bp	1.0	81.5
trnW-trnP_p5	GTTTTGGAGACCCACGTTCT	TGGGTACAAAATGTCACGGG	50.0	50.0	58.0	58.1	245 bp	1.0	81.5