Hotspot

psaI-ycf4

LSC · 59328-59729 · 402 bp

Back to Scorzonera

Pi 0.0149

MarkerSeek score 50.80

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 402
variable_sites: 6
pi: 0.0149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	ACAGAAAATCCGCCCCTTTT	ATAAAAAGACCCGCGATCCC	45.0	50.0	58.0	58.0	1315-1320 bp	1.0	81.8
psaI-ycf4_p2	ACAGAAAATCCGCCCCTTTT	ACGAGTCAATGGAATGGCTC	45.0	50.0	58.0	58.0	1556-1561 bp	1.0	81.5
psaI-ycf4_p3	GCAATCGCAATGGCTTCTTT	ATAAAAAGACCCGCGATCCC	45.0	50.0	58.0	58.0	683-684 bp	1.0	80.8
psaI-ycf4_p4	GCAATCGCAATGGCTTCTTT	ACGAGTCAATGGAATGGCTC	45.0	50.0	58.0	58.0	924-925 bp	1.0	80.5
psaI-ycf4_p5	GCAATCGCAATGGCTTCTTT	CCAGGAAATCCCCAACGAAA	45.0	50.0	58.0	58.1	788-789 bp	1.0	80.3