Hotspot

atpA-trnR-UCU

LSC · 29606-29746 · 141 bp

Back to Scorzonera

Pi 0.0426

MarkerSeek score 75.10

Primer available yes

Label atpA-trnR-UCU

Feature Metadata

label_name: atpA-trnR-UCU
feature_type: igs
region: LSC
length_bp: 141
variable_sites: 6
pi: 0.0426
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-trnR-UCU_p1	CATTCACCGAGGAAGCAGAA	GGTTCAAATCCTATTGGACGC	50.0	47.6	57.9	57.3	249-258 bp	1.0	67.6
atpA-trnR-UCU_p2	AAGACATTCACCGAGGAAGC	GGTTCAAATCCTATTGGACGC	50.0	47.6	57.9	57.3	253-262 bp	1.0	67.5
atpA-trnR-UCU_p3	TACTTCAGGAACAAGCAGCC	GGTTCAAATCCTATTGGACGC	50.0	47.6	57.8	57.3	186-195 bp	1.0	67.5
atpA-trnR-UCU_p4	CAGGAACAGAGGGAACGTTT	GGTTCAAATCCTATTGGACGC	50.0	47.6	57.7	57.3	208-217 bp	1.0	66.7
atpA-trnR-UCU_p5	ATTCACCGAGGAAGCAGAAG	GGTTCAAATCCTATTGGACGC	50.0	47.6	57.6	57.3	248-257 bp	1.0	65.2