Hotspot

trnE-UUC-rpoB

LSC · 12101-12898 · 798 bp

Back to Scorzonera

Pi 0.0177

MarkerSeek score 60.90

Primer available yes

Label trnE-UUC-rpoB

Feature Metadata

label_name: trnE-UUC-rpoB
feature_type: igs
region: LSC
length_bp: 798
variable_sites: 14
pi: 0.0177
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-rpoB_p1	GGGACTTGAACCCTCGTTTT	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	58.0	56.8	940-962 bp	1.0	63.7
trnE-UUC-rpoB_p2	TAACCACTGGACCATAGGGG	AGTTCTTCTGTCAAACCCTGA	55.0	42.9	58.1	56.8	899-921 bp	1.0	63.1
trnE-UUC-rpoB_p3	CCCTCGTTTTCTCCGTGAAA	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	58.1	56.8	930-952 bp	1.0	62.9
trnE-UUC-rpoB_p4	TTGAACCCTCGTTTTCTCCG	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	58.1	56.8	935-957 bp	1.0	62.9
trnE-UUC-rpoB_p5	GGGACTTGAACCCTCGTTTT	TCAAACCCTGATCAATGAACCT	50.0	40.9	58.0	57.6	930-952 bp	1.0	62.3