Hotspot

trnC-GCA-petN

LSC · 9511-10289 · 779 bp

Back to Scorzonera

Pi 0.0104

MarkerSeek score 57.20

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 779
variable_sites: 8
pi: 0.0104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	827-835 bp	1.0	79.7
trnC-GCA-petN_p2	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	845-853 bp	1.0	77.5
trnC-GCA-petN_p3	ATTATGTATCGTGGCGGCAT	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.5	58.2	881-889 bp	1.0	76.5
trnC-GCA-petN_p4	TTATGTATCGTGGCGGCATG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.5	58.2	880-888 bp	1.0	76.3
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACTAT	50.0	50.0	58.1	57.1	826-834 bp	1.0	73.7