Hotspot

trnK-UUU-rps16

LSC · 4388-5242 · 855 bp

Back to Scorzonera

Pi 0.0243
MarkerSeek score 63.30
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
855
variable_sites
20
pi
0.0243
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGATGGTATGGACGAATCC TGCCCTAATCAAACGAAATTCA 55.0 36.4 57.9 56.6 951-977 bp 1.0 56.4
trnK-UUU-rps16_p2 CGATGGTATGGACGAATCCC TGCCCTAATCAAACGAAATTCA 55.0 36.4 57.9 56.6 950-976 bp 1.0 56.4
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT TGCCCTAATCAAACGAAATTCA 50.0 36.4 58.2 56.6 890-916 bp 1.0 56.0
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TGCCCTAATCAAACGAAATTCA 50.0 36.4 58.2 56.6 889-915 bp 1.0 56.0
trnK-UUU-rps16_p5 TCCTAGCCGCACTTAAAAGC TGCCCTAATCAAACGAAATTCA 50.0 36.4 58.3 56.6 905-931 bp 1.0 55.2