Hotspot

ycf1

SSC · 125477-131101 · 5625 bp

Back to Scorpiothyrsus

Pi 0.0109

MarkerSeek score 51.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5625
variable_sites: 92
pi: 0.0109
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCCATGCTTCCGCTATTTCT	GTGCCCAAAGACGTAAGACA	50.0	50.0	58.0	58.1	267 bp	1.0	81.2
ycf1_p2	TCCCATGCTTCCGCTATTTC	GTGCCCAAAGACGTAAGACA	50.0	50.0	58.0	58.1	268 bp	1.0	81.1
ycf1_p3	CCCATGCTTCCGCTATTTCT	TCATCAAAGGTTCCATGCGT	50.0	45.0	58.0	57.8	285 bp	1.0	80.4
ycf1_p4	TCCCATGCTTCCGCTATTTC	TCATCAAAGGTTCCATGCGT	50.0	45.0	58.0	57.8	286 bp	1.0	80.4
ycf1_p5	CCCATGCTTCCGCTATTTCT	AGACAAACATCGGGTCGTTT	50.0	45.0	58.0	57.8	252 bp	1.0	80.3