Hotspot

rps15-ycf1

SSC · 125118-125476 · 359 bp

Back to Scorpiothyrsus

Pi 0.0325

MarkerSeek score 64.90

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 359
variable_sites: 16
pi: 0.0325
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AACACCATGCATTGATGGGT	TATCGAACCTGTGCGTTTGT	45.0	45.0	58.1	57.8	1322-1369 bp	1.0	81.4
rps15-ycf1_p2	TGCATTGATGGGTGATGAGG	TATCGAACCTGTGCGTTTGT	50.0	45.0	57.9	57.8	1315-1362 bp	1.0	81.4
rps15-ycf1_p3	GCATTGATGGGTGATGAGGT	TATCGAACCTGTGCGTTTGT	50.0	45.0	57.9	57.8	1314-1361 bp	1.0	81.4
rps15-ycf1_p4	TTGCTCGGGTCCATTTACAG	TATCGAACCTGTGCGTTTGT	50.0	45.0	57.9	57.8	1534-1558 bp	0.666667	64.9
rps15-ycf1_p5	GCTCGGGTCCATTTACAGTT	TATCGAACCTGTGCGTTTGT	50.0	45.0	57.9	57.8	1532-1556 bp	0.666667	64.8