Hotspot

rpl14-rps3

LSC · 82291-84028 · 1738 bp

Back to Scorpiothyrsus

Pi 0.00961

MarkerSeek score 59.80

Primer available yes

Label rpl14-rps3

Feature Metadata

label_name: rpl14-rps3
feature_type: igs
region: LSC
length_bp: 1738
variable_sites: 25
pi: 0.00961
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rps3_p1	CGCGACATTCAAATGGGTTT	CTCTACAAACCATTCGGGCT	45.0	50.0	57.7	57.6	1849-1872 bp	1.0	76.1
rpl14-rps3_p2	CGCGACATTCAAATGGGTTT	CAGTCCGAACGATCTATGGG	45.0	55.0	57.7	57.6	1807-1830 bp	1.0	75.9
rpl14-rps3_p3	CGCGACATTCAAATGGGTTT	CCTCTACAAACCATTCGGGC	45.0	55.0	57.7	58.6	1850-1873 bp	1.0	74.3
rpl14-rps3_p4	CACATCAATTTTCGAGCCCC	CTCTACAAACCATTCGGGCT	50.0	50.0	57.4	57.6	1878-1901 bp	1.0	74.3
rpl14-rps3_p5	CACATCAATTTTCGAGCCCC	CAGTCCGAACGATCTATGGG	50.0	55.0	57.4	57.6	1836-1859 bp	1.0	74.1