Hotspot

petD-rpoA

LSC · 78907-79142 · 236 bp

Back to Scorpiothyrsus

Pi 0.0254

MarkerSeek score 68.00

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 236
variable_sites: 9
pi: 0.0254
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	CATTTCGTCGTCCAGTAGCA	TCAGAAGCGATTTACCGAAGA	50.0	42.9	58.0	57.1	375-390 bp	1.0	67.3
petD-rpoA_p2	GGTTGGGTATTGGAGCAACA	TCAGAAGCGATTTACCGAAGA	50.0	42.9	58.1	57.1	318-333 bp	1.0	67.2
petD-rpoA_p3	TGGTTGGGTATTGGAGCAAC	TCAGAAGCGATTTACCGAAGA	50.0	42.9	58.1	57.1	319-334 bp	1.0	67.2
petD-rpoA_p4	TTGATTGGTACTGTGGTGGC	TCAGAAGCGATTTACCGAAGA	50.0	42.9	58.1	57.1	343-358 bp	1.0	66.9
petD-rpoA_p5	CGTCCAGTAGCAACAACTGT	TCAGAAGCGATTTACCGAAGA	50.0	42.9	58.1	57.1	367-382 bp	1.0	66.4