Hotspot

psbT-psbN

LSC · 75343-75414 · 72 bp

Back to Scorpiothyrsus

Pi 0.0926

MarkerSeek score 69.00

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 72
variable_sites: 10
pi: 0.0926
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	CCGCCGAAAGTTCCAACTAA	ATCCATTCGAAGAACACGGG	50.0	50.0	58.5	58.0	131 bp	1.0	82.0
psbT-psbN_p2	CCGCCGAAAGTTCCAACTAA	TTCGAAGAACACGGGGACTA	50.0	50.0	58.5	58.4	126 bp	1.0	79.4
psbT-psbN_p3	TTTTTCGAGAACCGCCGAA	ATCCATTCGAAGAACACGGG	47.4	50.0	58.0	58.0	142 bp	1.0	77.4
psbT-psbN_p4	CCGCCGAAAGTTCCAACTA	ATCCATTCGAAGAACACGGG	52.6	50.0	57.8	58.0	131 bp	1.0	76.1
psbT-psbN_p5	CCGCCGAAAGTTCCAACTAA	TCGAAGAACACGGGGACTAG	50.0	55.0	58.5	58.8	125 bp	1.0	75.9