Hotspot

ndhK-ndhC

LSC · 50988-51041 · 54 bp

Back to Scorpiothyrsus

Pi 0.037

MarkerSeek score 63.60

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 54
variable_sites: 3
pi: 0.037
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	TTTGGGTTGTTCGAGCAAGT	CATGGCGAAAAGGAGCATTG	45.0	50.0	58.2	58.1	129-132 bp	1.0	79.2
ndhK-ndhC_p2	TGGGTTGTTCGAGCAAGTAA	CATGGCGAAAAGGAGCATTG	45.0	50.0	57.4	58.1	127-130 bp	1.0	76.2
ndhK-ndhC_p3	TTGGGTTGTTCGAGCAAGTA	CATGGCGAAAAGGAGCATTG	45.0	50.0	57.4	58.1	128-131 bp	1.0	76.2
ndhK-ndhC_p4	TTTTGGGTTGTTCGAGCAAG	CATGGCGAAAAGGAGCATTG	45.0	50.0	57.1	58.1	130-133 bp	1.0	74.3
ndhK-ndhC_p5	TGGGTTGTTCGAGCAAGTAAA	CATGGCGAAAAGGAGCATTG	42.9	50.0	58.1	58.1	127-130 bp	1.0	72.6