Hotspot

ycf3

LSC · 43830-45831 · 2002 bp

Back to Scopolia

Pi 0.00452

MarkerSeek score 49.90

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 2002
variable_sites: 9
pi: 0.00452
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	AGGATAATAAATAAACGGGGTCTTCT	TCCCACGAGAGGTAATGAGA	34.6	50.0	58.0	57.2	2061-2074 bp	1.0	40.9
ycf3_p2	AGGATAATAAATAAACGGGGTCT	TCCCACGAGAGGTAATGAGA	34.8	50.0	54.9	57.2	2061-2074 bp	1.0	40.9
ycf3_p3	AGGATAATAAATAAACGGGGTCTTC	TCCCACGAGAGGTAATGAGA	36.0	50.0	56.7	57.2	2061-2074 bp	1.0	40.9
ycf3_p4	GGATAATAAATAAACGGGGTCTTCT	TCCCACGAGAGGTAATGAGA	36.0	50.0	56.7	57.2	2060-2073 bp	1.0	40.9
ycf3_p5	AGGATAATAAATAAACGGGGTCTTCT	TCCCACGAGAGGTAATGAGAA	34.6	47.6	58.0	57.9	2061-2074 bp	1.0	40.9