Hotspot

ycf1

SSC · 126169-131865 · 5697 bp

Back to Scopolia

Pi 0.00423

MarkerSeek score 43.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5697
variable_sites: 24
pi: 0.00423
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGCTTGGTATAATCCACGGT	AGGCCTGGGTTAAGATCGAT	45.0	50.0	56.6	58.2	282 bp	1.0	68.5
ycf1_p2	TGCTTGGTATAATCCACGGT	AGGTATTGAGGCCTGGGTTA	45.0	50.0	56.6	57.7	290 bp	1.0	67.8
ycf1_p3	TGCTTGGTATAATCCACGGTT	AGGCCTGGGTTAAGATCGAT	42.9	50.0	57.4	58.2	282 bp	1.0	66.5
ycf1_p4	TTGCTTGGTATAATCCACGGT	AGGCCTGGGTTAAGATCGAT	42.9	50.0	57.4	58.2	283 bp	1.0	66.5
ycf1_p5	TGCTTGGTATAATCCACGGTT	AGGTATTGAGGCCTGGGTTA	42.9	50.0	57.4	57.7	290 bp	1.0	65.8