Hotspot

rpoC2

LSC · 16840-21009 · 4170 bp

Back to Scopolia

Pi 0.00168

MarkerSeek score 54.80

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4170
variable_sites: 7
pi: 0.00168
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TATGGAAGAAGATCCGGGCA	GCCCTGTTCAGGTATCTCGA	50.0	55.0	58.2	58.9	268 bp	1.0	72.2
rpoC2_p2	TATGGAAGAAGATCCGGGCA	TGAACTAATGCCCTGTTCAGG	50.0	47.6	58.2	57.9	277 bp	1.0	70.7
rpoC2_p3	TATGGAAGAAGATCCGGGCA	GAACTAATGCCCTGTTCAGGT	50.0	47.6	58.2	57.9	276 bp	1.0	70.7
rpoC2_p4	TATGGAAGAAGATCCGGGCA	TGCCCTGTTCAGGTATCTCG	50.0	55.0	58.2	59.2	269 bp	1.0	69.9
rpoC2_p5	TATGGAAGAAGATCCGGGCA	AACTAATGCCCTGTTCAGGT	50.0	45.0	58.2	56.7	275 bp	1.0	69.1