Hotspot

rps12-rps7

IRa · 142853-142905 · 53 bp

Back to Scopolia

Pi 0.0189

MarkerSeek score 63.30

Primer available yes

Label rps12-rps7

Feature Metadata

label_name: rps12-rps7
feature_type: igs
region: IRa
length_bp: 53
variable_sites: 1
pi: 0.0189
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-rps7_p1	GGCTTGGAGGGAGATCTTTC	TTTTCTGCAGTACCTCGACG	55.0	50.0	57.7	57.9	148 bp	1.0	77.3
rps12-rps7_p2	TATGTACGGCTTGGAGGGAG	TTTTCTGCAGTACCTCGACG	55.0	50.0	58.6	57.9	155 bp	1.0	75.3
rps12-rps7_p3	GTATGTACGGCTTGGAGGGA	TTTTCTGCAGTACCTCGACG	55.0	50.0	58.9	57.9	156 bp	1.0	73.0
rps12-rps7_p4	GCCGTATTCGATGAAAGTCG	TTTTCTGCAGTACCTCGACG	50.0	50.0	57.1	57.9	175 bp	1.0	72.7
rps12-rps7_p5	GCTTGGAGGGAGATCTTTCA	TTTTCTGCAGTACCTCGACG	50.0	50.0	56.9	57.9	147 bp	1.0	71.3