Hotspot

ndhD-psaC

SSC · 119308-119425 · 118 bp

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Pi 0.0169

MarkerSeek score 62.20

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 118
variable_sites: 2
pi: 0.0169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AAGGAACCCGCAAATATCGG	CGCCTGTCCAACTGATTTCT	50.0	50.0	58.3	58.2	256 bp	1.0	76.5
ndhD-psaC_p2	AAGGAACCCGCAAATATCGG	AAGAGATGTGAATCCGCCTG	50.0	50.0	58.3	57.7	270 bp	1.0	75.4
ndhD-psaC_p3	AAGGAACCCGCAAATATCGG	CAGAGGACTGTGTTGGTTGT	50.0	50.0	58.3	57.7	290 bp	1.0	75.3
ndhD-psaC_p4	AAGGAACCCGCAAATATCGG	GAGATGTGAATCCGCCTGTC	50.0	55.0	58.3	58.4	268 bp	1.0	74.6
ndhD-psaC_p5	AAGGAACCCGCAAATATCGG	ATGGCATGAAACAACTCGCA	50.0	45.0	58.3	58.8	217 bp	1.0	72.1