Hotspot

ccsA-ndhD

SSC · 117565-117804 · 240 bp

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Pi 0.0208

MarkerSeek score 64.50

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 240
variable_sites: 5
pi: 0.0208
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ATTCTGCAATTGTAGCGGCT	ACCCGGATTTCGTTCTTTCA	45.0	45.0	58.2	57.4	427 bp	1.0	74.5
ccsA-ndhD_p2	TTCTGCAATTGTAGCGGCTA	ACCCGGATTTCGTTCTTTCA	45.0	45.0	57.6	57.4	426 bp	1.0	73.3
ccsA-ndhD_p3	AATTCTGCAATTGTAGCGGC	ACCCGGATTTCGTTCTTTCA	45.0	45.0	57.4	57.4	428 bp	1.0	72.0
ccsA-ndhD_p4	TCTGCAATTGTAGCGGCTAT	ACCCGGATTTCGTTCTTTCA	45.0	45.0	57.4	57.4	425 bp	1.0	71.5
ccsA-ndhD_p5	ATTCTGCAATTGTAGCGGCT	ACCCGGATTTCGTTCTTTCAT	45.0	42.9	58.2	57.9	427 bp	1.0	70.4