Hotspot

ycf15-trnL-CAA

IRb · 95958-96305 · 348 bp

Back to Scopolia

Pi 0.0144

MarkerSeek score 61.80

Primer available yes

Label ycf15-trnL-CAA

Feature Metadata

label_name: ycf15-trnL-CAA
feature_type: igs
region: IRb
length_bp: 348
variable_sites: 5
pi: 0.0144
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf15-trnL-CAA_p1	AGGAGAAGGAAGGCTGGAAT	AAATGGTAGACACGCGAGAC	50.0	50.0	58.0	58.0	474 bp	1.0	80.6
ycf15-trnL-CAA_p2	AGGAGAAGGAAGGCTGGAAT	TGAAATGGTAGACACGCGAG	50.0	50.0	58.0	58.0	476 bp	1.0	80.6
ycf15-trnL-CAA_p3	GGAGAAGGAAGGCTGGAATG	AAATGGTAGACACGCGAGAC	55.0	50.0	58.0	58.0	473 bp	1.0	80.4
ycf15-trnL-CAA_p4	GGAGAAGGAAGGCTGGAATG	TGAAATGGTAGACACGCGAG	55.0	50.0	58.0	58.0	475 bp	1.0	80.4
ycf15-trnL-CAA_p5	AGGAGAAGGAAGGCTGGAAT	GCCTTGGTGGTGAAATGGTA	50.0	50.0	58.0	58.1	486 bp	1.0	80.0