Hotspot

ndhC-trnV-UAC

LSC · 52201-53323 · 1123 bp

Back to Scopolia

Pi 0.00363

MarkerSeek score 62.70

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1123
variable_sites: 4
pi: 0.00363
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TTGCTAATCGGGGCTAACAC	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.0	57.8	1252-1263 bp	1.0	79.8
ndhC-trnV-UAC_p2	GCTAATCGGGGCTAACACTC	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.1	57.8	1250-1261 bp	1.0	79.0
ndhC-trnV-UAC_p3	CCTTTGCTAATCGGGGCTAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	1255-1266 bp	1.0	77.3
ndhC-trnV-UAC_p4	TTGCTAATCGGGGCTAACAC	GAAGGTCTACGGTTCGAGTC	50.0	55.0	58.0	57.5	1251-1262 bp	1.0	77.1
ndhC-trnV-UAC_p5	GGCTAACACTCCGGAAATGA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.6	57.8	1241-1252 bp	1.0	76.9