Hotspot

trnG-trnR

LSC · 9962-10121 · 160 bp

Back to Scopolia

Pi 0.0187

MarkerSeek score 63.30

Primer available yes

Label trnG-trnR

Feature Metadata

label_name: trnG-trnR
feature_type: igs
region: LSC
length_bp: 160
variable_sites: 3
pi: 0.0187
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-trnR_p1	AGCCTTCCAAGCTAACGATG	ACAGTCGTTCTTGCTATCGA	50.0	45.0	58.0	56.7	324 bp	1.0	70.1
trnG-trnR_p2	CCTAGCCTTCCAAGCTAACG	ACAGTCGTTCTTGCTATCGA	55.0	45.0	58.1	56.7	327 bp	1.0	69.9
trnG-trnR_p3	AGCCTTCCAAGCTAACGATG	ACAGTCGTTCTTGCTATCGAA	50.0	42.9	58.0	57.4	324 bp	1.0	67.9
trnG-trnR_p4	CCTAGCCTTCCAAGCTAACG	ACAGTCGTTCTTGCTATCGAA	55.0	42.9	58.1	57.4	327 bp	1.0	67.7
trnG-trnR_p5	CCCTAGCCTTCCAAGCTAAC	ACAGTCGTTCTTGCTATCGA	55.0	45.0	57.7	56.7	328 bp	1.0	67.7