Hotspot

trnK-rps16

LSC · 4363-5052 · 690 bp

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Pi 0.0522

MarkerSeek score 73.70

Primer available yes

Label trnK-rps16

Feature Metadata

label_name: trnK-rps16
feature_type: igs
region: LSC
length_bp: 690
variable_sites: 36
pi: 0.0522
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-rps16_p1	CCTTTCAGGATCAGTCGTGG	AAAAGGGTATGTTGCTGCCA	55.0	45.0	58.0	58.0	1521-1527 bp	1.0	81.8
trnK-rps16_p2	CCCTTTCAGGATCAGTCGTG	AAAAGGGTATGTTGCTGCCA	55.0	45.0	58.0	58.0	1522-1528 bp	1.0	81.7
trnK-rps16_p3	TCCCTTTCAGGATCAGTCGT	AAAAGGGTATGTTGCTGCCA	50.0	45.0	58.1	58.0	1523-1529 bp	1.0	81.4
trnK-rps16_p4	TCAGGATCAGTCGTGGTCTT	AAAAGGGTATGTTGCTGCCA	50.0	45.0	58.1	58.0	1517-1523 bp	1.0	81.3
trnK-rps16_p5	TTCAGGATCAGTCGTGGTCT	AAAAGGGTATGTTGCTGCCA	50.0	45.0	58.1	58.0	1518-1524 bp	1.0	81.3