Hotspot

rpl22

LSC · 83941-84330 · 390 bp

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Pi 0.00256

MarkerSeek score 42.60

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 390
variable_sites: 1
pi: 0.00256
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	TTGTCCCATAATCCCCCACT	ATTAAGAGTAAGAGGTAAACCC	50.0	36.4	58.0	52.6	525 bp	1.0	40.2
rpl22_p2	TTTGTCCCATAATCCCCCAC	ATTAAGAGTAAGAGGTAAACCC	50.0	36.4	57.2	52.6	526 bp	1.0	40.2
rpl22_p3	TGTCCCATAATCCCCCACTA	ATTAAGAGTAAGAGGTAAACCC	50.0	36.4	57.1	52.6	524 bp	1.0	40.2
rpl22_p4	TTTTGTCCCATAATCCCCCAC	ATTAAGAGTAAGAGGTAAACCC	47.6	36.4	57.9	52.6	527 bp	1.0	40.2
rpl22_p5	TTGTCCCATAATCCCCCACTA	ATTAAGAGTAAGAGGTAAACCC	47.6	36.4	57.8	52.6	525 bp	1.0	40.2